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. CRK12:CYC9 interact in a yeast two-hybrid assay and sort an active protein kinase complicated in procyclic and bloodstream variety T. bruceiTo analyze the subcellular localization of the CRK12 protein, a pEarleyGate104 vector was utilized for a transient expression in the CRK12 protein fused to yellow fluorescent protein (YFP). The confocal illus